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KMID : 0381120190410060647
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Genes and Genomics
2019 Volume.41 No. 6 p.647 ~ p.655
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Karyotype and B chromosome variation in Lilium amabile Palibin
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Nguyen Truong Xuan
Kim Bo-Ram
Park Doo-Ri
Kim Young-Kyu
Nguyen Viet-Yen
Na Jong-Kook
Kim Nam-Soo
Kim Jong-Hwa
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Abstract
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Objective: Lilium amabile Palibin (2n?=?2x?=?24) is an endemic lily species in Korea. B chromosomes are supernumerary chromosomes and the presence of B chromosome in L. amabile was known by previous researches. The current research was conducted to characterize the genetical and cytological features of the B chromosome plants in L. amabile.
Methods: Karyotype and B chromosome cytotype analyses were carried out among 135 L. amabile accessions that were collected from six geographical locations in Korea using conventional aceto-carmine staining as well as FISH technique with ribosomal RNA gene probes.
Results: The karyotype of L. amabile genome consisted of two large metacentric, four intermediate subtelocentric, and six intermediate to small acrocentric chromosomes in which chromosomes 1, 6 and 7 carried the 45S rRNA gene loci and chromosome 3 carried the 5S rRNA gene. There were 4 types of B chromosomes, two large B chromosomes and two small B chromosomes. The ribosomal RNA gene loci were not present in the B chromosomes. The 135 accessions were classified into 13 cytotypes including diploids and different B chromosome aneuploids. Among the aneuploids, the most frequent cytotype was 24?+?1B, which was followed by 24?+?2B, 24?+?1b, 24?+?1B?+?2b, 24?+?1B?+?4b, and 24?+?2B?+?4b.
Conclusion: The karyotype of L. amabile was consistent with other species in the genus Lilium without polyploids. The B chromosome cytotypes were highly variable and the occurrences of different cytotypes were random among the six populations, implying that the B and b chromosome occurrence was random in each population.
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KEYWORD
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Lilium amabile, Karyotype, B chromosome, FISH, Cytotypes
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